Isolation Of Flavonoid Compouns From The Leave Of The Mistletoe Host Jengkol Tree
Keywords:
Jengkol mistletoe, TLC, Column chromatography, FlavonoidsAbstract
Mistletoe is a parasitic plant that attaches itself to other plants to extract nutrients. It can be used
as a traditional medicine because this plant contains an active compound known as flavonoids.
Antibacterial activity tests were conducted on flavonoid compounds. The Isolation of flavonoid compounds
began by soaking dried mistletoe powder for 24 consecutively using hours using solvents n-hexane, ethyl
acetate and methane. The obtained extract was then concentrated using a rotary vacuum evaporator.
Flavonoid tests were conducted using the FeCl3 5% reagent and NaOH 10%. Spot test with thin layer
chromatography used solvent ratios of hexane: chloroform 3:2, 1:1, 2:3, 1:4 and chloroform: ethyl acetate
3:2, 1:1, 2:3, 1:4, 1:5 , 1:7, and 1:9. The Solvent that could separate the spots on the TLC was chloroform:
ethyl acetate = 2:3 (2 fractions), 1:4 (2-fractions), 1:5 (2 fractions), and 1:9 (4 fractions of the 6th fraction
in A single stain was obtained with Rf = 0.49 using the eluent chloroform ethyl acetate 1: 5. The 6th
fraction was concentrated using a vacuum rotary evaporator to obtain a concentrated extract with a deep
green color weighing 1.5 g.
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